P-5: Effect of Chronic Stress on Kiss-1 mRNA Expression in Male Rat Arcuate Hypothalamic Nucleus

Authors

  • A Ramezani Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
  • A Tamadon Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • B Namavar Jahromi Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • F Sabet Sarvestani Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • M Karami Kheirabad Department of Basic Sciences, Azad University, Gachsaran branch, Gachsaran, Iran
  • O Koohi-Hosseinabadi Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • S Ahmadloo Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

Background Kisspeptin is a potent stimulator of the hypothalamic- pituitary-gonadal axis and may play a critical role in modulating of stress-induced suppression of reproduction. The aim of the present study was to investigate the effect of glucocorticoid receptor antagonist (RU486) and stress on expression of Kiss-1 mRNA in arcuate nucleus (ARC) of rat hypothalamus. MaterialsAndMethods Twenty four male rats of the Sprague-Dawley strain were randomly allocated into stress, RU-486, stress/RU-486, and control groups (n=6). The stress group rats were immobilized 1 h/day, for 12 days through wrapping of their upper and lower limbs by packing tape with holes for ventilation. RU-486 (2.5 mg/kg, subcutaneously) for 12 days was injected into the RU-486 group rats. The stress/ RU-486 group rats were injected with the same dose of RU- 486 1 hours before the stress process for 12 days. The control group was allowed to freely move in the cages without receiving any stress or drug. All rats were decapitated and their ARC was collected. Moreover, five rats were ethically killed 2 weeks after castration and served as real time PCR control group. Relative expression of Kiss-1 mRNAs (compared to the castrated group) in ARC was determined using qualitative real-time PCR. Mean of data in four groups were compared by one-way ANOVA and LSD post-hoc test (SPSS 22; P

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Expression of RFamide-Related Peptide-3 (RFRP-3) mRNA in Dorsomedial Hypothalamic Nucleus and KiSS-1 mRNA in Arcuate Nucleus of Rat during Pregnancy

full text

P-21: RF-Amide Related Peptide mRNA Expression in Male Rat Dorsomedial Hypothalamic Nucleus during Chronic Stress

Background Chronic stress induces male reproductive dysfunction; however, the involved mechanisms are not clear. It has been suggested that the gonadotrophin-inhibitory hormone, known as RF-amide related peptide (RFRP) in mammalian species, may mediate this inhibitory effect of stress. The objective of the present study was to compare the effect of chronic stress and glucocorticoids receptor an...

full text

Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus

Objective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pup...

full text

expression of rfamide-related peptide-3 (rfrp-3) mrna in dorsomedial hypothalamic nucleus and kiss-1 mrna in arcuate nucleus of rat during pregnancy

0

full text

increased litter size and suckling intensity inhibit kiss-1 mrna expression in rat arcuate nucleus

objective(s): the effect of litter size and suckling intensity on the expression of kiss-1 mrna in the arcuate nucleus (arc) of rats were evaluated. materials and methods: thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. lactating rats were allotted to eight equal groups. in three groups, litter size was adjusted to 5, 10, or 15 pup...

full text

Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus

OBJECTIVES The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. MATERIALS AND METHODS Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 9  issue 2

pages  45- 46

publication date 2015-09-01

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023